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Plasmid#119895PurposeFLP(D) with a hsp70 promoterDepositorInsertFLP(D)
UseDrosophila expressionTagsExpressionMutationmutated form of FLP, which at position 5 contains…PromoterAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Zyxin-6
Plasmid#57441PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertZyxin (ZYX Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLIK TT 3xFLAG LacZ hygro
Plasmid#98395PurposeLentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tagDepositorInsertLacZ
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mesp-1916/-1>nls::Cas9::nls
Plasmid#59988PurposeMesp driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Mesp -1916/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_Psyn-sgRNA500
Plasmid#149557PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsyn and pSynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_Psyn-mCherryBb
Plasmid#149636PurposePsyn-mCherry promoter strength reporter for use in B. burgdorferiDepositorInsertPsyn-mCherryBb
UseTagsmCherryExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-TAX-L10-STEPtag
Plasmid#159375PurposeExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertTAX-L10-STEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag-L10-TAX
Plasmid#159374PurposeExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertSTEPtag-L10-TAX
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV-Flag-mPar6a.PIG
Plasmid#24599DepositorInsertPar6c
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H08
Plasmid#70996PurposeGateway entry cloneDepositorInsertNap1 (Nap1 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
dFP
Plasmid#120542PurposeExpresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.DepositorInsertde novo fluorescence-activating protein (dFP)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRS306Sup35R1+2
Plasmid#17716DepositorInsertSup35R1+2 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFUS2_b4
Plasmid#50698PurposeIntermediate array vectorDepositorInsertLacZ + BsaI restriction sites
UseTALENTagsExpressionMutationPromoterAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T merlin 340-590
Plasmid#11633DepositorInsertmerlin (NF2 Human)
UseTagsGSTExpressionBacterialMutationCarboxy-terminal half of merlin isoform 2.PromoterAvailable sinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Flag-mPar6aK19A
Plasmid#24598DepositorInsertPar6c
UseRetroviralTagsFLAGExpressionMammalianMutationK19APromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SpCas9
Plasmid#107328PurposeU6 driven SpCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_NmCas9
Plasmid#107329PurposeU6 driven NmCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -