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Plasmid#80923PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hsyn-GRAB_HA1m
Plasmid#190474PurposeExpress the medium-affinity histamine sensor GRAB_HA1m in neuronsDepositorInsertHistamine sensor GRAB_HA1m (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-PML-IV
Plasmid#62804Purposeexpresses the isoform IV of PML (promyelocytic leukemia protein)DepositorInsertPML-IV (PML Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-NEDD8
Plasmid#19943DepositorInsertNEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_empty_gRNA
Plasmid#197557Purposesites for cloning two gRNA with puromycin cassetteDepositorTypeEmpty backboneUseEmpty grna backbone with puromycin resistance geneTagsExpressionMammalianMutationPromoterU6 for gRNA, CMV for puromycin resistance.Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1mut
Plasmid#190475PurposeExpress the histamine-insensitive control sensor GRAB_HA1mut in neuronsDepositorInsertHistamine-insensitive sensor GRAB_HA1mut (control sensor)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hNINJ1-GFP
Plasmid#208779PurposeInducibly expresses human NINJ1 tagged with GFPDepositorInsertNINJ1 (NINJ1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(K310R)
Plasmid#23250DepositorInsertNFkB RelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationK310RPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
UseTagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ TREX1-KI
Plasmid#127701PurposeFor knocking in TREX1 in mouse - Doxycyclin inducibleDepositorInsertmouse TREX1 cDNA (Trex1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET26b-Granzyme A (S-A)
Plasmid#8825DepositorInsertHuman Granzyme A inactive (GZMA Human)
UseTags(His)6ExpressionBacterialMutationchanged Serine 184 to AlaninePromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane EGFP (LCK Synthetic, Human, A. victoria)
UseSea urchin, mammal, zebrafishTagsEGFPExpressionMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC236
Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC235
Plasmid#216372PurposexhNup155-Nb3i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb3i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only