Addgene

We narrowed to 6,532 results for: 11

Showing: 3721 - 3740 of 6532 results

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoM

Availability

Academic Institutions and Nonprofits only

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PatoM-LOG241

Plasmid

#72847

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoM

Availability

Academic Institutions and Nonprofits only

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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rubicon

Plasmid

#21636

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Rubicon (1700021K19Rik Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*(see below)

Promoter

Availability

Academic Institutions and Nonprofits only

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ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

Plasmid

#21863

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codons between the colinear uORF1 and uORF2 …

Promoter

Availability

Academic Institutions and Nonprofits only

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pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb RORgamma-t mut

Plasmid

#20127

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Tags

Expression

Mutation

RORgamma-t binding site in 0.9kb of 2kb promoter …

Promoter

Availability

Academic Institutions and Nonprofits only
G608G lamin A minigene

Plasmid

#20292

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G606G:GGC>GGT Mutation causes aberrant spli…

Promoter

Availability

Academic Institutions and Nonprofits only
MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Promoter

Availability

Academic Institutions and Nonprofits only

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pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Use

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Promoter

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

Plasmid

#140197

Purpose

Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

D832A DNase deactivated

Promoter

PgpdA

Availability

Academic Institutions and Nonprofits only

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pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

Promoter

PgpdA

Availability

Academic Institutions and Nonprofits only

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pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

Plasmid

#140205

Purpose

Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Inserts

mCherry
sgRNA E1

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Tags

Expression

Mutation

G174D

Promoter

PelcA and U3

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot3_239-590_K

Plasmid

#146796

Purpose

Mammalian Expression of HsNot3_239-590

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_239-590 (CNOT3 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Promoter

Availability

Academic Institutions and Nonprofits only

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