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Plasmid#79724PurposeThis plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1E (CSNK1E Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3_HUMAN_D0
Plasmid#79721PurposeThis plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDAPK3 (DAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT MEK5-DD-mRFP1
Plasmid#47547PurposeGateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutantDepositorInsertMEK5-DD (MAP2K5 Human)
UseEntry vectorTagsmRFPExpressionMutationS311D, T315DPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rabbit Cav 2.2 W391A pMT2
Plasmid#58734Purposeexpression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2DepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RVR
Plasmid#108863PurposeFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutationsDepositorInsertFnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R, K629R and N633R, Cpf1 is rice codon optimi…PromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:Beta2meGFP
Plasmid#45096DepositorInsertnAChR beta2 (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationThere are strain differences between C57BJ/6 and …PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK11_HUMAN_D0
Plasmid#79692PurposeThis plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK11 (MAPK11 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FES_HUMAN_D0
Plasmid#79686PurposeThis plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFES (FES Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK3_HUMAN_D0
Plasmid#79702PurposeThis plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMAPK3 (MAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xHA-MKK6-EE
Plasmid#47544PurposeGateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutantDepositorInsert3xHA-MKK6-EE (MAP2K6 Human)
UseEntry vectorTags3XHAExpressionMutationS207E, T211EPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR153
Plasmid#226808PurposeGateway entry vector for 2xHA GPR153DepositorInsertGPR153 (GPR153 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_997253.2PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA LTB4R
Plasmid#226806PurposeGateway entry vector for 2xHA LTB4RDepositorInsertLTB4R (LTB4R Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin
Plasmid#223965PurposeDual fluorescent reporter for histone H2B and for Cdt1DepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM 1995
Plasmid#217826PurposeExpresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.DepositorInsertHY4 (CRY1 Mustard Weed)
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 W391A pcDNA3
Plasmid#206085Purposeexpression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-ERBB2 NLS
Plasmid#192316PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLSDepositorInsertmCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-HsNRF2 (NFE2L2)
Plasmid#194302PurposeMultisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRSDepositorInsertNFE2L2 (NFE2L2 Human)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-MmCavin4
Plasmid#170528PurposeEntry clone for tagging MmCavin4 at the C terminus. Parton lab clone ADMDepositorInsertCavin4 (Cavin4 Mouse)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-MmCav1
Plasmid#170527PurposeEntry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJDepositorInsertCaveolin1 (Cav1 Mouse)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ288 (BoCas12a)
Plasmid#138125PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoterDepositorInsertBoCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only