Addgene

We narrowed to 4,639 results for: codon optimized

Showing: 3701 - 3720 of 4639 results

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pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

Plasmid

#164845

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

Depositor

Erik Procko

Article

Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Use

Tags

8his tag and HA leader sequence

Expression

Mammalian

Mutation

Codon-optimized for human cell expression. Has N5…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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EGFP-COV E

Plasmid

#165123

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV E (E codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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EGFP-COV M

Plasmid

#165124

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV M (M codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Nsp4 deltaC-EGFP

Plasmid

#165126

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp13 K289A-EGFP

Plasmid

#165130

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET-PE2-His (IK1822)

Plasmid

#170103

Purpose

T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

Depositor

Keith Joung

Article

Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis

Use

CRISPR

Tags

6xHis tag

Expression

Bacterial

Mutation

Bacteria codon-optimized nSpCas9 (H840A) & M-…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM9,10

Plasmid

#170280

Purpose

Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

Insert

mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

Availability

Academic Institutions and Nonprofits only

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pGLOW39

Plasmid

#173068

Purpose

mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

mScarlet-I-C1^SEC^3xMyc

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mScarlet

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171380

Purpose

Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized RfxCas13d

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, U6

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp1

Plasmid

#156465

Purpose

Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3a_Ub1

Plasmid

#156467

Purpose

Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3b_ADRP

Plasmid

#156468

Purpose

Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3b_ADRP (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3c_SUD-C

Plasmid

#156471

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-C (ORF1ab Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3d_Ub2_PLPro

Plasmid

#156472

Purpose

Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3d_Ub2_PLPro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp4

Plasmid

#156474

Purpose

Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

Enlarge