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pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pOT2 3GLA

Plasmid

#71764

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2H4

Plasmid

#67094

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H4 (GTF2H4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
TAL2320

Plasmid

#36746

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Left (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2321

Plasmid

#36747

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Right (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
A337C/Y344C

Plasmid

#24434

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Use

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
gabra5_R (TAL3015)

Plasmid

#36007

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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gabra5_L (TAL3014)

Plasmid

#36006

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3 V460P

Plasmid

#78814

Purpose

To overexpress RIPK3 V460P in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

V460P

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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K222C/E334C

Plasmid

#24435

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Use

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL G457D

Plasmid

#61367

Purpose

Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with G457D mutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed G457 to D

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL N464D

Plasmid

#61369

Purpose

Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with N464D mutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed N464to D

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
K420CLM RP

Plasmid

#24443

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Use

Tags

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pMTH PKC gamma

Plasmid

#8412

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
M71::Myc6-IRES-taulLacZ LNL TV

Plasmid

#15619

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only