-
Plasmid#173873PurposeMammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]
Plasmid#40807DepositorInsertMAP2K1 (MAP2K1 Human)
UseTagsHAExpressionMammalianMutationdelta(44-51), S218E, S222DPromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
3580 pcDNA3 Bad S112A S136A
Plasmid#8799DepositorInsertBad (Bad Mouse)
UseTagsExpressionMammalianMutationS112A, S136APromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4
Plasmid#58316PurposeFLAG-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Plxna4-Fc-His
Plasmid#72125PurposeExpresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxna4 (Plxna4 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173868PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PAK7_HUMAN_D0
Plasmid#79706PurposeThis plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK7 (PAK5 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
UseTagsGFPExpressionBacterial and YeastMutationPromoterPRC1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA
Plasmid#37756PurposeExpresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Synthetic, Human)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGZ173 (pFA6a-SL-MNase-kanMX6)
Plasmid#70234PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsertShort linker and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC8_GWs-Lox (VE5589)
Plasmid#163769PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.DepositorInsertno insert
UseTagsno tagExpressionInsectMutationPromoterPHAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-S473A
Plasmid#49190PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationS473APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]
Plasmid#40806DepositorInsertMAP2K2 (MAP2K2 Human)
UseTagsHAExpressionMammalianMutationdelta(48-55) + S222D + S226DPromoterMSV-LTRAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shGAC
Plasmid#110410PurposeshGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.DepositorInsertGLS glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only