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Plasmid#8757DepositorInsertMcl-1 cDNA PCR fragment (Mcl1 Mouse)
UseTagsExpressionBacterialMutationBase pairs 943-1223.PromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/Full length FVIII
Plasmid#41036DepositorInsertFVIII (F8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab7A
Plasmid#61803PurposeHuman Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorInsertRab7A (RAB7A Human)
UseTags2x HA and GFPExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD3
Plasmid#24167PurposeMammalian expression of human JMJD3 with HA tagDepositorInsertJMJD3 (KDM6B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Tag5Amyc-GSK3b WT
Plasmid#16260DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ADAM17-HA
Plasmid#65105PurposeExpression of HA tagged ADAM17 (TACE) in mammalian cellsDepositorInsertADAM17 (ADAM17 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/BDD-FVIII
Plasmid#41035DepositorInsertB domain deleted FVIII (F8 Human)
UseTagsExpressionMammalianMutationB domain deletionPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA-Akt DN (K179M)
Plasmid#16243DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (K179M)PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-63: ACTN2-mEGFP
Plasmid#124607PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCa-C1
Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorInsertprotein kinase C alpha (PRKCA Bovine)
UseTagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCa-mEGFP-N1
Plasmid#112269PurposeIDOCKSalpha Donor ConstructDepositorInsertProtein Kinase C alpha (PRKCA Bovine)
UseTagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseTagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-WT(pL84)
Plasmid#124400PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.DepositorInsertGal4-TET1-878-911 wildtype (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a)
Plasmid#79672PurposeCre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP (Scn9a jellyfish, Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only