-
Plasmid#174858PurposeExpresses recoded mouse Peg10 in mammalian cellsDepositorInsertrecoded Mus musculus PEG10 (Peg10 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Green Pegassos
Plasmid#163114PurposeExpresses biosensors for pyruvate in mammalian cellsDepositorInsertGreen Pegassos
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Green Lindoblum
Plasmid#163113PurposeExpresses biosensor for lactate in mammalian cellsDepositorInsertGreen Lindolum
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL3718 (pQCascade_Pse)
Plasmid#200899PurposeExpresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.DepositorInsertPseCas7, PseCas8, PseCas6, PseTniQ
UseTagsNLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD1
Plasmid#195839PurposeExpresses human FZD1 with an N-terminal HiBiTDepositorInsertFZD1 (FZD1 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorInsertKlotho (KL Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
Klotho (membrane)
Plasmid#17712DepositorInsertKlotho (KL Human)
UseTagsV5ExpressionMammalianMutationP101SPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorInsertrecoded Homo sapiens PEG10 (PEG10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cas9)
Plasmid#174864PurposeExpresses human Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by HsPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-4A/4B-CAAX
Plasmid#87699PurposeExpress mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cellsDepositorInsertmCherry-4A/4B-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Long P2A
Plasmid#164111PurposeDetect the long Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Short P2A
Plasmid#164112PurposeDetect the short Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
UseTags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-Sumo1 WT
Plasmid#48966PurposeExpresses HA-Sumo1 WT in mammalian cellsDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHAExpressionMammalianMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pc3.1-CAGGS-ASAP3-mCyRFP3
Plasmid#193323PurposeA red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3DepositorInsertASAP3-R3
UseTagsExpressionMammalianMutationPromotercaggsAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only