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A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker