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Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV 5TOP intron renilla TRICK CTE polyA
Plasmid#64544Purpose5′ TOP TRICK reporter mRNADepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, 5' TOP (5′ terminal oligo…ExpressionMammalianMutationPromoterTetracycline-inducible promoter and 5′ UTR of hum…Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2/GD-IRES-GFP-CTNNB1
Plasmid#192868PurposeCarries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.DepositorInsertsluc+
EGFP
CTNNB1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorInsertCUL1 3'UTR (CUL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-miR126-EGFL7-Promoter
Plasmid#32244DepositorUseLuciferaseTagsExpressionMammalianMutationPromoter region 5' UTRPromoter5' UTRAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSB118 - pL2_pSB90_fLUC-I_HcPro
Plasmid#123193Purposebinary plant vector for transient TEV HcPro and fLUC (with intron) expressionDepositorInsert2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 (-756/+68) SBR2 Mut 3' box
Plasmid#15711DepositorInsertp15 promoter (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMut 3' box in SBR2. The 3' box contains…PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB116 - pL2_pSB90_fLUC-I_P19
Plasmid#123191Purposebinary plant vector for transient TBSV P19 and fLUC (with intron) expressionDepositorInsert2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p190-FLAG
Plasmid#205620PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p190 isoform b3a2
UseTagsFLAGExpressionMammalianMutationT117M- Please see depositor commentsPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_1-3
Plasmid#210629PurposeLuciferase experiment for TAL1 promoter 1-3DepositorInsertpromoter 1-3 (TAL1 Human)
UseTagsExpressionMammalianMutationPromoterMinimal PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
proE-cad670-Luc-mEbox
Plasmid#42084DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains E-cadherin promoter region from -670 to …PromoterE-cadherinAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114103Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
Volvox Channelrhodopsin 1
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E_inD)Luc
Plasmid#200109PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element in front of EGR1 promoter…Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DICER-Prom
Plasmid#25851DepositorInsertDicer promoter (DICER1 gene ID 23405, Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses the Gluc-iLID fusion protein in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Vim/Actb5UTR-renilla
Plasmid#38238DepositorUseLuciferaseTagsExpressionMammalianMutationActb promoter (1000 nt upstream) and 5' UTR …PromoterEndogenous VimAvailable sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
Plasmid#192939PurposeFlpO-based digitizer circuit driven by AP1 promoterDepositorInsertsAP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP2A
Plasmid#128349PurposeVector for LUMIER assayDepositorInsertPP2A (PTPA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only