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Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W
Plasmid#109416PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R46Q
Plasmid#109414PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR46Q repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_N99D
Plasmid#109410PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationN99D repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW:PtU6
Plasmid#104895PurposePhaeodactylum U6 promoterDepositorInsertPhaeodactylum U6 promoter
UseTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACBE-ASR (pXK-607)
Plasmid#208822PurposeUniversal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterCMV, U6Available sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Immune checkpoint (library 2) Backbone
Plasmid#192363PurposePlasmid vector for in vivo immune checkpoint screening library (for checkpoint library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
T cell activation (library 1) Backbone
Plasmid#192364PurposePlasmid vector for in vitro Jurkat activation screening (for immune library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_K102E_E115K
Plasmid#109405PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationK102E and E115K repA mutations to increase copy n…PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_N99K
Plasmid#109409PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationN99K repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_K102E
Plasmid#109408PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationK102E repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_E115K
Plasmid#109407PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationE115K repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_M78I
Plasmid#109413PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationM78I repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam
Plasmid#160451Purposevector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRFHUE-eGFP
Plasmid#89469PurposeGFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformationDepositorInserteGFP
UseBinary plasmid for atmt of filamentous fungiTagsExpressionMutationPromoterA. nidulans gpdAAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only