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Plasmid#104570PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domainsDepositorInsertdLbCpf1(D832A)-DmrA(x3)
UseTags3x FLAG, 3x HA, and NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1D4-mCherry
Plasmid#73423PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.DepositorInsertPromoter 1D4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1D4 (orthogonal T7-lac variant)Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4A6-mCherry
Plasmid#73424PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.DepositorInsertPromoter 4A6 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4A6 (orthogonal T7-lac variant)Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1E4-mCherry
Plasmid#73426PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.DepositorInsertPromoter 1E4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1E4 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1B6-mCherry
Plasmid#73420PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.DepositorInsertPromoter 1B6 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1B6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3H5-mCherry
Plasmid#73419PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.DepositorInsertPromoter 3H5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3H5 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL035
Plasmid#122854PurposeG9a SET domain with GreenGate D-E flanking sequencesDepositorInsertG9a SET catalytic domain, codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL023
Plasmid#122841Purposep300 core domain with GreenGate D-E flanking sequencesDepositorInsertCore domain of p300 H3K27 histone acetyltransferase, codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorTagsExpressionMutationContains D10A and H840A mutationsPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN1A-c001
Plasmid#175491PurposeProtein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.DepositorInsertCAPN1 / CAPNS1 (CAPN1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN2A-c001
Plasmid#175492PurposeProtein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.DepositorInsertCAPN2 / CAPNS1 (CAPN2 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-opEBNA2-IRES-mCherry
Plasmid#220355PurposeExpresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)DepositorInsertopEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralTagsExpressionMammalianMutationcodon-optimised for expression in mammalian cellsPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)
Viral Prep#51669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFG.wtCNb_opt.IRES.eGFP
Plasmid#22492DepositorInsertCodon optimised wild type Calcineurin B (PPP3R1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pICSL80005
Plasmid#50322DepositorInsertCDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNb30_opt.IRES.eGFP
Plasmid#22493DepositorInsertCodon optimised Calcineurin B mutant 30 (PPP3R1 Human)
UseRetroviralTagsExpressionMutationL124T; K125-LA-InsPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPF144
Plasmid#106372PurposeC. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusADepositorInsertPcwp2-gusA (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPcwp2Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID6A
Plasmid#165046PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only