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Bacterial Strain#201049PurposeDH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.DepositorBacterial ResistanceStreptomycinAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDJSVT87
Plasmid#109302PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tagDepositorTypeEmpty backboneUseTagsOmpA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT89
Plasmid#109303PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneUseTagsTamA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT90
Plasmid#109304PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneUseTagsTamA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AS+
Plasmid#78242PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS-
Plasmid#78245PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 BS+
Plasmid#78244PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AS-
Plasmid#78243PurposeOne of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AD-uvr8at
Plasmid#44971DepositorInsertAD-UVR8 (UVR8 Mustard Weed)
UseTagsNF-κB activation domainExpressionMammalianMutationdeleted Methionine 1PromoterpCMVAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDYU1H-TED
Plasmid#154300PurposeA expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B-TED
Plasmid#154299PurposeA expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AN-
Plasmid#78247PurposeOne of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS4 AN+
Plasmid#78246PurposeOne of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).DepositorInsertsattB
attP
UseTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEXPYR
Plasmid#70030PurposeHigh yield protein secretion in Aspergillus nidulansDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterA. niger glucoamylase promoter (glaAp)4Available sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT91
Plasmid#109305PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tagDepositorTypeEmpty backboneUseTagsSufI 1-30, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcS-p88-C1C2
Plasmid#178424Purposep88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domainDepositorInsertp88
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDYU1G-TED
Plasmid#154298PurposeA expression vector in Dictyostelium discoideum containing the G418 resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL_FR_Hygro
Plasmid#127171PurposeFrameshift reporter (control plasmid for screening)DepositorInsertFrameshift Reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only