Showing: 341 - 360 of 363 results
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Plasmid#162503PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.DepositorUseTagsGFPExpressionMammalianMutationOne Tac stem region is inserted within ST6Gal1 an…PromoterAvailabilityAcademic Institutions and Nonprofits only
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ST-2xTstem(5A)-GFP
Plasmid#162504PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.DepositorUseTagsGFPExpressionMammalianMutationOne Tac stem region is inserted within ST6Gal1 an…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pmVirD2-GFP
Plasmid#90315PurposemVirD2-GFP expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204 - GFP
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7/T5 IPTG inducibleAvailabilityAcademic Institutions and Nonprofits only -
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Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mCer-Vamp2(qv/fw)
Plasmid#53238PurposeMutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationMutated Q76 to V; F77 to W.PromoterAvailabilityAcademic Institutions and Nonprofits only -
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SBP-ST*-GFP
Plasmid#163668PurposeExpresses SBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
FKBP-ST*-GFP
Plasmid#163669PurposeExpresses FKBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsFK506 binding protein (FKBP) and GFPExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem-GFP
Plasmid#162501PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe Tac stem region is inserted within ST6Gal1PromoterAvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem(5A)-GFP
Plasmid#162502PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe mutated Tac stem region is inserted within ST…PromoterAvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NAD-Snifit
Plasmid#113658PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentrationDepositorInsertNAD-Snifit
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-nucl
Plasmid#113915PurposePlasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NAD-Snifit-mito
Plasmid#113916PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorInsertNAD-Snifit
UseTagsExpressionMammalianMutationPromoterCMV TetO2AvailabilityAcademic Institutions and Nonprofits only
Showing: 341 - 360 of 363 results