ST-Tstem-GFP
(Plasmid
#162501)
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PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmoxGFP-N1
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Backbone manufacturerA gift from E. Snapp (Addgene plasmid # 68070)
- Backbone size w/o insert (bp) 4697
- Total vector size (bp) 6005
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameST6Gal1 mutant (Tac stem region is inserted within ST)
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SpeciesH. sapiens (human)
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Insert Size (bp)1311
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MutationThe Tac stem region is inserted within ST6Gal1
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GenBank IDBC040009.1 NM_000417.3
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Entrez GeneST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
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Entrez GeneIL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ST-Tstem-GFP was a gift from Lei Lu (Addgene plasmid # 162501 ; http://n2t.net/addgene:162501 ; RRID:Addgene_162501) -
For your References section:
Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314