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Plasmid#233156PurposeRetroviral expression of fluorescent mRuby2 reporterDepositorInsertBrn2-2A-mRuby2 (Pou3f2 Mouse, Synthetic)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-Mad-mRuby2
Plasmid#233239PurposeVinculin FRET tension sensor with coiled-coil Mad domain no vinculin force controlDepositorInsertVinculin-Clover-CC-Mad-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
Plasmid#229800PurposeBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1aDepositorInsertmGreenLantern; mRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-IgK-pHluorin-TM-mRuby
Plasmid#185546PurposeExpresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoterDepositorInsertIgK-pHluorin-TM-mRuby
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-mRuby3-Pho8-406
Plasmid#207043PurposeExpression of mRuby3-Pho8 under CPU1 promoter.DepositorInsertPHO8
UseTagsmRuby3ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)
Plasmid#209776PurposeCRISPRi for STK4DepositorInsertNluc, sgSTK4i, mRuby2 (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-jAspSnFR3-mRuby3
Plasmid#203459PurposejAspSnFR3-mRuby3 in adenoviral expression vectorDepositorInsertjAspSnFR3
UseAAV and AdenoviralTagsHis-tag and mRuby3ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-jAspSnFR3-mRuby3-NLS
Plasmid#203489PurposeNuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vectorDepositorInsertjAspSnFR3-mRuby3-NLS
UseGateway entry vectorTagsNuclear localization signal and mRuby3ExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-MTS-jAspSnFR3-mRuby3
Plasmid#203490PurposeMitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vectorDepositorInsertMTS-jAspSnFR3
UseGateway entry vectorTagsHis-tag, Mitochondrial localization signal, and m…ExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304-CAG-jAspSnFR3-mRuby3
Plasmid#203500PurposejAspSnFR3-mRuby3 in lentiviral expression vectorDepositorInsertjAspSnFR3-mRuby3
UseLentiviralTagsHis-tag and mRuby3ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Blast-Dest-LifeAct-mRuby
Plasmid#194907PurposeTo generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.DepositorInsertLifeAct
UseMouse TargetingTagsmRubyExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mRuby3-WPRE
Plasmid#193336PurposeExpresses mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mRuby-Rab35
Plasmid#194334PurposeFor use in light-induced Rab35 protein inactivation through the interaction with CRY2DepositorUseTagsmRubyExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2389-Tier1-PhCMV-p2a_mRuby
Plasmid#169526PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).DepositorInsertPCMV-driven P2A-fused enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mRuby3-DDX21 HGV
Plasmid#179233Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmRuby3ExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-VHH[GFP]
Plasmid#163856PurposeZebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRXDepositorInsertVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mRuby2 L1-R5
Plasmid#84389PurposeMultisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmRuby2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPguCas13b-3xmRuby3-NLS
Plasmid#132406Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only