Addgene

We narrowed to 405 results for: lenticrispr

Showing: 341 - 360 of 405 results

Results per page:

Sort results by:

lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_P2A_Puro

Plasmid

#211364

Purpose

Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

Depositor

Jacques Neefjes

Article

Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-SpCas9 blast

Plasmid

#104997

Purpose

This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1

Plasmid

#208387

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

Plasmid

#208388

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

Plasmid

#181973

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-p300 HAT

Plasmid

#179544

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

Plasmid

#181972

Purpose

Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-CBP HAT

Plasmid

#179547

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-GCN5 core

Plasmid

#179551

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNluc-sgControl

Plasmid

#208383

Purpose

This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgControl (TP53BP1 intron) (Trp53bp1 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNLuc-sgmMRE11

Plasmid

#208384

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgMRE11 (Mre11a Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

Plasmid

#208389

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA-Cre-GpNLuc-sgmMLKL

Plasmid

#208391

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgMLKL (Mlkl Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-p300 PHD-HAT

Plasmid

#179545

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-CBP RING-PHD-HAT

Plasmid

#179549

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-CBP PHD-HAT

Plasmid

#179548

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-p300 RING-PHD-HAT

Plasmid

#179546

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 341 - 360 of 405 results

Results per page:

Sort results by: