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Plasmid#210404PurposeFor expression of VHH with C-terminal his and Flag tagsDepositorTypeEmpty backboneUseTagshis6-flagExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro
Plasmid#58492Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationdeleted amino acids P29 to D408 and bearing mutat…PromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_Luciferase
Plasmid#136528PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_3xflag_NRF2
Plasmid#136527PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#174666PurposeDoxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent proteinDepositorInsertArid3a-3xFLAG (Arid3a Mouse)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterTREAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_SOX2
Plasmid#45270DepositorInsertMCOPS3 (SOX2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pflaG
Plasmid#45477DepositorInsertflaA-GFP
UseTagsExpressionBacterialMutationPromoterflaAAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_P278A
Plasmid#183113PurposeExpresses flag-tagged p53 with P278A mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine, see depositor com…PromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PHC3
Plasmid#45262DepositorInsertPHC3 (PHC3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FLAG.Ollas.VSVg_NGFR
Plasmid#158324PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsFLAG.Ollas.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIP_RNA2FLAG
Plasmid#61261PurposeFlag tagged Orsay virus RNA2 genome segment expression plasmidDepositorInsertOrsay RNA2
UseTagsFlag fusedExpressionWormMutationPromoterAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
Plasmid#79902PurposeGene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor LocusDepositorInsert3xFLAG_Twin_Strep_EZH2
UseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterPGK1Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_Flag_NS3
Plasmid#79634PurposeThird generation Self-inactivating lentivector expressing NS3 from Zika virusDepositorInsertNS3
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myr.PKCzeta.FLAG
Plasmid#10802DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAG and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG.PKCzeta.T410E
Plasmid#10804DepositorInsertPKC zeta (Prkcz Rat)
UseTagsFLAGExpressionMammalianMutationActivation loop active: T410E (Thr 410 to Glu)PromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcihygroflagsynhDAT
Plasmid#19990DepositorInsertDopamine transporter (SLC6A3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_FNDC3B
Plasmid#45269DepositorInsertFNDC3B (FNDC3B Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_Flag_PrM
Plasmid#79632PurposeThird generation Self-inactivating lentivector expressing PrM from Zika virusDepositorInsertPrM
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1hLCAD_FLAG
Plasmid#24491DepositorInsertLong chain acyl-CoA dehydrogenase (ACADL Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only