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Plasmid#110832PurposeExpresses dTomato (dT) red fluorescent reporter.DepositorInsertdTomato Tag
UseNon-viral gene expression vectorTagsIRESExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA ZNHIT3
Plasmid#110953PurposeTransient expression of HA ZNHIT3DepositorInsertZNHIT3 (ZNHIT3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-GFP-PGK-Puro
Plasmid#110868PurposeLentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
E. coli 16S toehold switch sensor
Plasmid#110698PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. coli 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-Puro
Plasmid#110839PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAC1 (RAC1A-c007)
Plasmid#110257PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAC1 (RAC1 Human)
UseTagsStrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#45
Plasmid#110926PurposeVector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegansDepositorInsertshsp-16-2 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast shGAC
Plasmid#110410PurposeshGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.DepositorInsertGLS glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA Pol III)Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK124
Plasmid#110583PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP25 promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK102
Plasmid#110574PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertT7 promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
cfSBFP2-N
Plasmid#110633PurposeCys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.DepositorInsertcfSBFP2
UseTagsExpressionMutationCys48Ser/Cys70MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shGLS
Plasmid#110335PurposeshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selectionDepositorInsertGLS glutaminase (GLS Synthetic, Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#371
Plasmid#110882PurposeExpresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neuronsDepositorInsertsunc-129 promoter
CTNS-1a-mCherry cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsintron-mCherryExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK563
Plasmid#110612PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoterDepositorInsertNanoluc
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain intein/chitin binding domain (79-156)
Plasmid#110760PurposeBacterial expression of ISG15 C-term.DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN3
Plasmid#110637PurposeRetroviral expression of Flag-SFXN3 in mammalian cellsDepositorInsertSFXN3 (SFXN3 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL
Plasmid#110741PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGPD-luxAB(HIS)
Plasmid#1106DepositorInsertbacterial luciferase
UseTagsExpressionYeastMutationn/aPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KPNB1_HEAT
Plasmid#110017PurposeProtein expression and purification of KPNB1_HEATDepositorInsertKPNB1_HEAT (KPNB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only