Addgene

We narrowed to 4,006 results for: gast

Showing: 3561 - 3580 of 4006 results

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Use

Tags

6xHis and V5

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-CTNNB1_S33Y

Plasmid

#133376

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

CTNNB1 (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S33Y

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-Fc-His

Plasmid

#72189

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.23 EC10-Fc-His

Plasmid

#72187

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-AP-His

Plasmid

#72063

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn1-3 (CG11739)

Plasmid

#110642

Purpose

Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG11739 (CG11739 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Tags

Expression

Mutation

N-term deletion of first 46 amino acids, includes…

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Use

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-AP-His

Plasmid

#72064

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Dscam1_7.27.22 EC10-AP-His

Plasmid

#72060

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.22

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Dscam1_7.27.23 EC10-AP-His

Plasmid

#72061

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Dscam1_7.27.22 EC10-Fc-His

Plasmid

#72186

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.22

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H04

Plasmid

#70971

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf6 (Taf6 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only