Addgene

We narrowed to 49,071 results for: eng

Showing: 3561 - 3580 of 49071 results

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pBiT3.1-C_WDR89:1-387

Plasmid

#211049

Purpose

Mammalian expression of full-length human WDR89. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR89:M1-Q387 (WDR89 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SKIC8:M1-I305 (WDR61 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RBBP4:1-425

Plasmid

#211037

Purpose

Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP4:M1-S425 (RBBP4 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAKTaq

Plasmid

#25712

Purpose

Bacterial expression of Taq polymerase

Depositor

David Engelke

Article

Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

Insert

Taq polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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BioBrick pET-24a(+)

Plasmid

#117802

Purpose

BioBrick pET vector for protein overexpression in E. coli

Depositor

Anthony Forster

Article

Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

Type

Empty backbone

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW 0690

Plasmid

#74123

Purpose

Photinus pyralis sulfotransferase ST3 - pHis8-4

Depositor

Jing-Ke Weng

Article

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis sulfotransferase 3

Use

Tags

8x-His,TEV protease site (ENLYFQ)

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSCBE3

Plasmid

#133966

Purpose

Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

BE3

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJH110

Plasmid

#68376

Purpose

Clone cassette to replace RFP for integration into C31 landing pad strain

Depositor

Trevor Charles

Article

Heil et al J Vis Exp. 2012 Mar 16;(61). pii: 3698.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionRD

Plasmid

#172924

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSCBE2

Plasmid

#133965

Purpose

Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

BE2

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionRE

Plasmid

#172926

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBdxs

Plasmid

#17821

Depositor

Jay Keasling

Article

Kim et al Biotechnol Bioeng. 2001 Feb 20. 72(4):40

Insert

dxs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pJKW 0643

Plasmid

#74121

Purpose

Photinus pyralis luciferin sulfotransferase LST - pHis8-4

Depositor

Jing-Ke Weng

Article

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis firefly luciferin sulfotransferase

Use

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionKE

Plasmid

#172925

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorKE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFBR2

Plasmid

#116800

Purpose

Lentiviral expression of TGFBR2

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAC1-P29S

Plasmid

#116650

Purpose

Lentiviral expression of RAC1 P29S

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

P29S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD-E1021K

Plasmid

#116566

Purpose

Lentiviral expression of PIK3CD E1021K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mutation

E1021K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA

Plasmid

#116771

Purpose

Lentiviral expression of PIK3CA

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRB

Plasmid

#116770

Purpose

Lentiviral expression of PDGFRB

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRB (PDGFRB Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge