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Plasmid#149638Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, emptyDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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pCS2 hSmad1-EVE
Plasmid#22993DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS463E, S465EPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113342PurposeExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertdCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV4-ZIKV-NS4A
Plasmid#178845PurposeMammalian expression of ZIKV ORFDepositorInsertZIKV-NS4A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Casp2
Plasmid#50023PurposepENTR vector for mouse Caspase-2DepositorInsertCasp2 (Casp2 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113344PurposeExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-ΔF3
Plasmid#163618Purposemouse SNX27 without the F3 domain cDNA C-terminally tagged with GFPDepositorInsertmouse SNX27-ΔF3 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationdeletion F3 domainPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Neg. Ctrl_sgRNA
Plasmid#111298PurposeCRISPR KO murine neg. ctrl.DepositorInsertNegCtrl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF CAG mc2 IRES hygro
Plasmid#206234PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MsCLYBL_sgRNA1
Plasmid#111294PurposeCRISPR KO murine CLYBLDepositorInsertsgRNA1 against murine CLYBL
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PRLSS-SH2-KDEL
Plasmid#209716PurposeMammalian expression of SH2 localized to endoplasmic reticulumDepositorInsertPRLSS-SH2-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR
Plasmid#107266PurposemCherry-eDHFR in pIVT vector for in vitro transcriptionDepositorInsertmCherry-eDHFR (dhfr E.coli)
UseTagsmCherryExpressionMammalianMutationPromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SauCas9
Plasmid#174040PurposeIn vitro transcription of SauCas9 mRNADepositorInsertSauCas9
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A
Plasmid#163619Purposemouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112APromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only