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Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-Luc
Plasmid#25739PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP1A
Plasmid#128348PurposeVector for LUMIER assayDepositorInsertPP1A (PPP1CA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the Gluc-EL222 fusion protein in bacteriaDepositorInsertGaussia Luciferase - EL222
UseTagsExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic
Plasmid#32737DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL
Plasmid#65713PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL)DepositorInsert7xTcf, minCMV, 3xNLS, ONL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorInsertAkt1(D323A/D325A) (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TmiR-Luc
Plasmid#25743PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS
Plasmid#123189Purposebinary plant vector for transient fLUC (with intron) expressionDepositorInsert2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m2
Plasmid#15449DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationAll 3 Sp1 sites mutatedPromoterAvailable sinceAug. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(T308A/S473A)
Plasmid#86578PurposeExpresses human full-length Akt1(T308A/S473A) phosphomutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationT308A/S473A phosphomutantPromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
Plasmid#125229PurposeExpresses transmembrane intracellular NanoLuc in mammalian cellsDepositorInsertIgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic
Plasmid#32736DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorInsertFRB-Akt1(D274A) (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM1157
Plasmid#112534PurposepZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
luxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO) and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-B7-EYFP
Plasmid#114110Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only