Showing: 3541 - 3560 of 4276 results
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Plasmid#112148PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and SE…PromoterAvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
UseTags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
Plasmid#176027Purposeconstruct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometryDepositorInsertsrtTA3
Luc2
mtagBFP
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR2
Plasmid#176245PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR3
Plasmid#176246PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_CRATES
Plasmid#176251PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitplDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2
Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA Td1
Plasmid#176257PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-1
Plasmid#190095Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-1 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 6704-6709 refer to NM_0070…PromoterAvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-2
Plasmid#190096Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-2 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 7122-7129 refer to NM_0070…PromoterAvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR
Plasmid#186740PurposeDox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorUseLentiviralTagsRAB10-T2A-RABIF-P2A-mCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
lentiTet-CSK-P2A-mCherry BlastR
Plasmid#186742PurposeDox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertCSK (CSK Human)
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAR100
Plasmid#180377PurposepAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).DepositorInsertsNFAT-Luciferase
hCD8A
UseTagsExpressionBacterial and MammalianMutationPromoterhEF1-HTLV and minimal promoter and an NFAT respon…AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral mBmal1-Luc
Plasmid#182762PurposeLentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporterDepositorInsertmouse arntl promoter (Bmal1 Mouse)
UseLentiviralTagsExpressionMutationPromoter649 bpAvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40AvailabilityAcademic Institutions and Nonprofits only
Showing: 3541 - 3560 of 4276 results