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Plasmid#177124PurposeExpress Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-Runx1+23
Plasmid#69602PurposeRunx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloningDepositorInsertsUse5' gateway entry vectorTagsExpressionMutationPromoterC57/BL6 mouse Runx1 +23 intronic enhancer and C57…Available sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-MBP-TEV
Plasmid#105771PurposeExpression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsMBP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S_nostop
Plasmid#152988PurposeGateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV_iRFP_P2AT2A_mScarlet-I_Myl9
Plasmid#172474PurposeMammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.DepositorInsertsUseLentiviralTagsmScarlet-IExpressionMammalianMutationPromoterEF1alpha (with UCOE) and none (same transcript as…Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-EGFP
Plasmid#105793PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCherry-TEV
Plasmid#105779PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsmCherry-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCherry
Plasmid#105796PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-FLAG-BirA-TEV
Plasmid#105770PurposeExpression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsFLAG-BirA-TEVExpressionMammalianMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF3B
Plasmid#141272PurposeGateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF3B
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)
Plasmid#139447PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop
Plasmid#154029PurposeGateway-compatible Entry vectorDepositorInsertNSP3
UseGateway-compatible entry vector, with mutagenized…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-TEV-ProteinA
Plasmid#105788PurposeExpression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-ProteinAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tight promoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)
Plasmid#139443PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-hDND1
Plasmid#70070PurposeGateway entry vector for human DND1 with stop codonDepositorInsertDnd1 (DND1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-mRuby3-TEV
Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-eGFP
Plasmid#122033PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only