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pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C from figure 4A of paper.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation I from figure 4A of paper.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Publication

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Pdgfra-T2A-H2B-eGFP donor

Plasmid

#113120

Purpose

Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_HMOX1-V5-APEX2

Plasmid

#85823

Purpose

Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Publication

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

HMOX1-V5-APEX2 (HMOX1 Synthetic, Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-GFP-PQRv3-RFP

Plasmid

#73951

Purpose

Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

Depositor

Brian Chen

Publication

Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

Inserts

sfGFP (superfolder GFP)
RFP

Tags

A residual PQR peptide wii be left at the C-termi…

Expression

Mammalian

Promoter

Chicken beta actin and Chicken beta actin (shared…

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only
pLV.SP146.gp91.eGFP.cHS4

Plasmid

#30476

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP/cHS4

Use

Lentiviral

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1_CD

Plasmid

#83571

Purpose

Expresses a mutant catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Publication

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted, catalytic domain…

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

Plasmid

#10881

Depositor

Patrick Aebischer

Publication

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1mismatch (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
3543 pMIG Bcl-XL mut8

Plasmid

#8792

Depositor

Stanley Korsmeyer

Publication

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G138A, R139L, I140N

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Publication

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_4

Plasmid

#26706

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Par3 RNAi

Available since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
M20 siamois-luciferase p01234

Plasmid

#17157

Depositor

Randall Moon

Publication

Brannon et al Genes Dev. 1997 Sep 15. 11(18):2359-70.

Insert

siamois promoter (sia1.L Frog)

Use

Luciferase

Tags

luciferase

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Publication

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Publication

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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