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pCJH009_proD_Cas12c-sgRNA-GFP_CAM

Plasmid

#183070

Purpose

Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c single guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm2_A

Plasmid

#145881

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm3_A

Plasmid

#145882

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm5_A

Plasmid

#145884

Purpose

Insect Expression of DmLSm5

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm5 (CG6610 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm7_A

Plasmid

#145885

Purpose

Insect Expression of DmLSm7

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm7 (LSm7 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSV40L

Plasmid

#165798

Purpose

Viral Simian virus 40 Late poly(a) terminator element

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SV40L

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPromoterless-P2A

Plasmid

#165790

Purpose

Viral P2A part in promoter grammar, for building promoterless control vectors

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless-2A

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p3xP3-Hsp70b

Plasmid

#165773

Purpose

HSP70B Promoter fused to synthetic 3xP3 eye driver

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3x3-Hsp70b

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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5xGMR

Plasmid

#165775

Purpose

5xGMR syntethic eye driver enhancer, requires minimal promoter element

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xGMR

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-hph

Plasmid

#165879

Purpose

Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-pA_U6-BbsI (pE057)

Plasmid

#149351

Purpose

Mammalian expression vector for Venus

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miRFP670

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Shark-tSH2

Plasmid

#126672

Purpose

Expression of Shark tandem SH2 domains SNAP tagged for use as FRET reporter

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark tandem SH2 domains

Expression

Bacterial

Available since

July 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMT Draper-ITAM only-GFP

Plasmid

#126656

Purpose

Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe

Expression

Insect

Mutation

Cytoplasmic ITAM residues Tyr all others mutated …

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Plasmid

#137118

Purpose

Protease Switch Clone ID: 2-B3

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Use

Synthetic Biology

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Publication

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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