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Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_SMU1:1-513
Plasmid#211114PurposeMammalian expression of full-length human SMU1. N-terminal HiBiT tag.DepositorInsertSMU1:M1-P513 (SMU1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0527-luc2
Plasmid#214657Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0527
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2353
Plasmid#144017PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF12 (ZNF12 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSExpressionMutationE174R, S542R, K548RPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1-5xmut_O
Plasmid#147116PurposeMammalian Expression of HsNxf-1-5xmutDepositorInsertHsNxf-1-5xmut (NXF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA
Plasmid#108735PurposeExpresses N-terminus to aa256 of Cre recombinase fused to FRB CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertUB2" (SNRPB2 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_ChR2_mCherry
Plasmid#213937PurposeAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherryDepositorInsertChannelrhodopsin 2-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB_rtTA_BsmBI
Plasmid#126028PurposePiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloningDepositorTypeEmpty backboneUseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterU6Available sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB19c-GFP
Plasmid#126139PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR
Plasmid#28235PurposeMammalian expression of Androgen receptor fused to EGFPDepositorInsertAndrogen receptor (AR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-D1-IRES-SS-D2-IRES-HA-D3
Plasmid#215119PurposeExpresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.DepositorUseLentiviralTagsFLAG-tag, HA-tag, and SS (STREP-STREP)ExpressionMammalianMutationPromoterIRES and TREG3Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only