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Plasmid#11206DepositorInsertNedd4-1 (Nedd4 Mouse)
UseTagsHAExpressionMammalianMutationcysteine 744 mutated to glutamic acidPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-Nedd4/2795
Plasmid#11426DepositorInsertNedd4-1 (Nedd4 Mouse)
UseTagsHAExpressionMammalianMutationD661N compared to GenBank reference sequence U966…PromoterCMVAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 8
Plasmid#36106DepositorInsertG protein subunit Gamma 8 (GNG8 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
PLK1-cjun-FRET-PACT biosensor
Plasmid#106907Purposecentrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)DepositorInsertPLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
UseTagsPACT domainExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH (TB40/E)
Plasmid#136446PurposeMammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-lengthDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2
Plasmid#169531PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).DepositorInsertPCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
untargeted Plk1 FRET sensor Myt1 substrate
Plasmid#45217DepositorInsertuntargeted Plk1 FRET sensor Myt1 substrate
UseTagsExpressionMammalianMutationPromotert7Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Histone H2B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX
Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1
Plasmid#10830DepositorInsertArf1 (ARF1 Bovine)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smac(1-60)mCherry
Plasmid#40880DepositorInsertSmac(1-60)mCherry (DIABLO Human)
UseTagsmCherryExpressionMutationPromoterCMVAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 WT
Plasmid#198201PurposeFRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenusDepositorInsertMYO5A IQ domain (Myo5a Mouse)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CENP-B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45214DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…PromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PhoCl-mCherry-myc
Plasmid#87692PurposeExpress PhoCl-mCherry with C-term myc tag in mammalian cellsDepositorInsertPhoCl-mCherry-myc
UseTagsmyc tagExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Stra6L-6His
Plasmid#174215PurposeStra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.DepositorInsertStra6L (Stra6l Mouse)
UseTags6-HisExpressionMammalianMutationnonPromoterCMVAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX
Plasmid#188992PurposeA plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only