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Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRAK1mut-UTR
Plasmid#15096DepositorInsertIRAK1 3'UTR (IRAK1 Human)
UseLuciferaseTagsExpressionMammalianMutation4-bp substitutions in the miR-146 target sitesPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA15 (GNA15 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position E(244)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
Plasmid#72888Purposefusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase
Channelrhodopsin-2
enhanced yellow fluorescent protein
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast
Plasmid#213826PurposeExpresses human PRDM6 with a C-terminal V5 epitope tag.DepositorInsertPRDM6 (PRDM6 Human)
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAF6mut-UTR
Plasmid#15094DepositorInsertTRAF6 3'UTR (TRAF6 Human)
UseLuciferaseTagsExpressionMammalianMutation4-bp substitutions in the miR-146 target sitesPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-STAT1 5'-UTR
Plasmid#115353Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of STAT1DepositorInsertSTAT1 5'-UTR (STAT1 Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
phFluc-WT
Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOPO tTR-KRAB-rtTA3-Luc
Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
R26TV LSL-TRL
Plasmid#68476PurposeRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassetteDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseCre/Lox, Luciferase, and Mouse TargetingTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TGmiR-Luc
Plasmid#25745PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMIR-CDH1 wtUTR
Plasmid#25038DepositorInsertCDH1 3'UTR (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hEZH2
Plasmid#112543Purpose3'UTR reporter assayDepositorInsertEZH2 (EZH2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
-227 hTF (mutant NF-kB)
Plasmid#15443DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationNF-kappaB binding domain mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-ZF5.3-GFP-HiBit
Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only