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Plasmid#177854PurposeBacterial Expression of D14DepositorInsertD14
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a His6 GST PYL2(14-188)
Plasmid#177842PurposeBacterial Expression of PYL2DepositorInsertPYR1-like 2 (PYL2 Mustard Weed)
UseTags6xHis-GSTExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-Strep-His E314Amb
Plasmid#169021PurposeExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHis and SBPExpressionBacterialMutationE314TAGPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_dead_mt
Plasmid#167270PurposeOverexpression of the dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAP-tag
UseTagsHis x10ExpressionBacterialMutationC145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast_dead_mt
Plasmid#167272PurposeOverexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
UseTagsHis x10ExpressionBacterialMutationE30R-C145APromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3567 (pET151_Spt6 1247-1451 R1282H)
Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3495 (pET151_Spt6 1247-1451 K1435A)
Plasmid#91817PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1435 to alanine; deleted residues …PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (GB1 WT - A34F/V39L)
Plasmid#136610PurposeExpresses GB1 WT double mutant (A34F/V39L)DepositorInsertGB1 WT double mutant (A34F/V39L)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: A34F - V39LPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-3XNLS-WPRE
Plasmid#159950Purposenucleus markerDepositorInsertYpet-3XNLS
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-optoJNKi-WPRE
Plasmid#159967Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activationDepositorInsertYpet-optoJNKi
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(+7A)-IDR-mCherry
Plasmid#145285PurposeBacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion ProteinDepositorInsertHOXA13(+7A)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXA13(WT)-IDR-mCherry
Plasmid#145284PurposeBacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion ProteinDepositorInsertHOXA13(WT)-IDR (HOXA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+7A)-IDR-mCherry
Plasmid#145281PurposeBacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104A/M247C)
Plasmid#155133PurposeExpresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/M247C, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
UseTags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF H407A H409A H448A
Plasmid#139566Purposeexpressed SznF H407A H409A H448A in E. coliDepositorInsertSznF H407A H409A H448A
UseTagshexahistidineExpressionBacterialMutationchanged glutamate 215 to alaninePromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-S98 (for p24)
Plasmid#130717PurposePlasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid proteinDepositorInsertEGFP-LAMA-S98
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only