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Plasmid#65679PurposeExpresses beta-tubulin-CNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_Golgi
Plasmid#65693PurposeExpresses Golgi-YNL in mammalian cellsDepositorInsertN-terminal 81 amino acids of beta 1,4-galactosyltransferase
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_ITPKA
Plasmid#65687PurposeExpresses ITPKA-YNL in mammalian cellsDepositorInsertinositol triphosphate 3-kinase A
UseLuciferaseTagsYNLExpressionMammalianMutationcontains residues 9-52PromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-C1_PTS1
Plasmid#65700PurposeExpresses CNL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_PTS1
Plasmid#65701PurposeExpresses ONL-PTS1 in mammalian cellsDepositorInsertperoxisomal targeting signal 1 (tripeptide SKL)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_Mito
Plasmid#65671PurposeExpresses Mito-ONL in mammalian cellsDepositorInsertcytochrome c oxidase subunit VIII
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Zyxin-6
Plasmid#57853PurposeLocalization: Focal Adhesions, Excitation: 548, Emission: 561DepositorInsertZyxin (ZYX Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-SiT-N-15
Plasmid#55508PurposeLocalization: Golgi TGN, Excitation: 462, Emission: 492DepositorInsertSiT (ST6GAL1 Human)
UseTagsmTFP1ExpressionMammalianMutationaa 1-45 of SiTPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Talin-ABM-10
Plasmid#55510PurposeLocalization: Talin Actin Binding Domain, Excitation: 462, Emission: 492DepositorInsertTalin ABM
UseTagsmTFP1ExpressionMammalianMutationActin Binding Module; I/LWEQ = 197 amino acids fr…PromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
refGFP1_pQE30
Plasmid#48644PurposeBact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFPDepositorInsertrefGFP1
UseTags6xHisExpressionBacterialMutationrefGFP C147S, C204QPromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Flag Smad3 AAGY
Plasmid#27027DepositorInsertSmad3 (SMAD3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationP181A P182APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-KDD359-361FSY
Plasmid#24578DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationLysine-Aspartate-Aspartate 359-361 changed to Phe…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-H2B-mGFP
Plasmid#173892Purposefor H2B labelingDepositorInsertH2B (H2BC21 Human)
UseLentiviralTagsmGFPExpressionMutationPromoterSFFVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WHaloCaMP1a-EGFP
Plasmid#205313PurposeExpression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoterDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-Flag-natT1R2
Plasmid#113944Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK18msB
Plasmid#177839PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2
Plasmid#161753PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.DepositorInsertExRai-AKAR2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only