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Showing: 3461 - 3480 of 3954 results

  1. pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag

    Plasmid
    #179977
    Purpose
    Mammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).
    Depositor
    Konstantin Sparrer
    Article
    Hayn et al Cell Rep. 2021 May 18;35(7):109126.
    Insert
    SARS-CoV-2 E (E Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179977-plasmid-map-sequence-id-353866

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  4. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

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  6. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  8. pET3a-SF-LMNC-TS

    Plasmid
    #169374
    Purpose
    E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    LMNA residues 31-542 (LMNA Human)
    Use
    Tags
    Split-FlAsH Tag and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-30 (unstructured head domain…
    Promoter
    Native pET3a promotor
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169374-plasmid-map-sequence-id-333772

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  9. pET21-10XHis-GST-HRV-Her1-dL5

    Plasmid
    #73216
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5 (EGFR Schistosoma japonicum, Human, Synthetic)
    Use
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73216-plasmid-map-sequence-id-137065

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  10. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Schistosoma japonicum, Human, Synthetic)
    Use
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

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  11. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  12. pCDH-V1AUC-DelStem-CD20-Puro

    Plasmid
    #209758
    Purpose
    Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.
    Depositor
    Andrei Thomas-Tikhonenko
    Article
    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:
    Insert
    MS4A1 (MS4A1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations to disrupt the uORFs and the stem-loop …
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    209758-plasmid-map-sequence-id-414085

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  13. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

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  15. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid
    #202199
    Purpose
    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination
    Depositor
    Ofer Yizhar
    Article
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Has Service
    AAV1 and AAV5
    Insert
    stChrimsonR, PdCO
    Use
    AAV and Cre/Lox
    Tags
    EGFP and Rho1D4
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202199-plasmid-map-sequence-id-397078

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  18. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

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  19. pcDNA3.3_SARS2_Mu

    Plasmid
    #177578
    Purpose
    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production
    Depositor
    David Nemazee
    Article
    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj
    Insert
    Spike (Mu variant) (S SARS-CoV-2)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177578-plasmid-map-sequence-id-348240

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Showing: 3461 - 3480 of 3954 results