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Plasmid#204173PurposepRegev_S4 with double mutations that restore secondary structure.DepositorInsertExon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.34C>A_46G>U
Plasmid#204161PurposepRegev_S1 with double mutations that restore secondary structure.DepositorInsertS1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>A and 46G>U mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.51A>C
Plasmid#204172PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 51A>C mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation51A>C mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G
Plasmid#204171PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D1
Plasmid#204178PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D1 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G.51A>C
Plasmid#204165PurposepRegev_S2 with double mutations that restore secondary structure.DepositorInsertExon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A
Plasmid#204175PurposepRegev_S5 with single mutation that disrupts secondary structure.DepositorInsertExon S5 with 49C>A mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D4
Plasmid#204184PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertD4 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D2
Plasmid#204180PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D2 in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation61C>G mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-jAspSnFR3-mRuby3-NLS
Plasmid#203489PurposeNuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vectorDepositorInsertjAspSnFR3-mRuby3-NLS
UseGateway entry vectorTagsNuclear localization signal and mRuby3ExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A.59G>U
Plasmid#204177PurposepRegev_S5 with double mutations that restore secondary structure.DepositorInsertExon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A and 59G>U mutationsPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_ExonSkip_Backbone
Plasmid#204187PurposeBackbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).DepositorInsertFirst exon and intron for beta globin splicing minigene
UseTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas12i2-T2A-EGFP
Plasmid#188270PurposeMammalian expression, Genome editingDepositorInsertCas12i2
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b-T2A-EGFP
Plasmid#188271PurposeMammalian expression, Genome editingDepositorInsertAaCas12b
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PlmCasX-T2A-EGFP
Plasmid#188273PurposeMammalian expression, Genome editingDepositorInsertPlmCasX
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-Cas12i2
Plasmid#188275Purposeprokaryotic expressionDepositorInsertCas12i2
UseCRISPRTagsExpressionBacterialMutationnonePromoterT7Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-Cas12i-Max
Plasmid#188276Purposeprokaryotic expressionDepositorInsertCas12i-Max
UseCRISPRTagsExpressionBacterialMutationnonePromoterT7Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(plus)
Plasmid#201149PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(plus)DepositorInserteGCaMP+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBP60g-NLS-3xmCitrine
Plasmid#192521PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertCALMODULIN BINDING PROTEIN 60g
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterCBP60gAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPop2a-V5His6_G
Plasmid#146390PurposeInsect Expression of DmPop2aDepositorInsertDmPop2a (Pop2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only