Addgene

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pDONR221-ARL3-no stop

Plasmid

#67321

Purpose

hArl3 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF1

Plasmid

#67306

Purpose

hArf1 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL1-no stop

Plasmid

#67317

Purpose

hArl1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL11

Plasmid

#67340

Purpose

hArl11 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_006009.2

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Pyl-TAG

Plasmid

#228784

Purpose

Pyl aaRS/tRNA system for mammalian TAG suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAG suppressor

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Pyl-TAA

Plasmid

#228783

Purpose

Pyl aaRS/tRNA system for mammalian TAA suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

PylRS
PytR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAA suppressor

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCF153_Gag-Pol

Plasmid

#225961

Purpose

CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

CMV-Intron-GagPol (FMLV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

Insert

U6-sgRNA (recipient)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Tags

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

Plasmid

#216276

Purpose

AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox

Tags

6XHIS and Xpress

Expression

Mutation

Promoter

human synapsin 1 (hSyn)

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E6

Plasmid

#213469

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cda-32aa linker-z…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E7

Plasmid

#213470

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd-32aa linker-z…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E8

Plasmid

#213471

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

Tags

No

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-mRuby3

Plasmid

#166136

Purpose

Gateway entry vector for mRuby3

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

mRuby3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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