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Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-RPA4/His14 (MSW#949)
Plasmid#208075PurposeExpression of human Replication Protein A 4 and His-tagged RPA3 in E.coliDepositorInsertRPA4, RPA14(His-tagged)
UseTags10xHis tag on RPA14ExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHis-SUMOExpressionBacterialMutationΔ37PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorInsertRab10 (RAB10 Human)
UseTagsHis-SUMOExpressionBacterialMutationQ68LPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaR-SpCas9-NLS-6xHis
Plasmid#207387PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-Sniper SpCas9-NLS-6xHis
Plasmid#207377PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-Sniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Blackjack SpCas9-NLS-6xHis
Plasmid#207375PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertBlackjack SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationamino acids 1005-1013 replaced with two glycinePromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-SARS-CoV-2-Nucleocapsid
Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-865)
Plasmid#203335PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-865 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-885)
Plasmid#203334PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-885 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a- phage MazG-like/Atd1
Plasmid#195244PurposeExpersses phage MazG-like/Atd1DepositorInsertphage MazG
UseTagsFlag and TwinStrepExpressionBacterialMutationCatalytic site mutantPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-20b_HtrA2(delta(133))_S306A_6His
Plasmid#197975PurposeE. coli expression of HtrA2(delta133)-6HisDepositorInsertHtrA2 (HTRA2 Human)
UseTags6HisExpressionBacterialMutationdelta(1-133), S306APromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-A602E_M
Plasmid#146964PurposeBacterial Expression of NcQde-2_506-938-A602EDepositorInsertNcQde-2_506-938-A602E
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only