Addgene

We narrowed to 23,004 results for: GFP

Showing: 3421 - 3440 of 23004 results

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EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-(L)-mApple

Plasmid

#182856

Purpose

Hetero-dimer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESN2_GFP_YTA_Champ1_328-354

Plasmid

#105639

Purpose

Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

Depositor

Thanos Halazonetis

Insert

Champ1 (CHAMP1 Human)

Use

Tags

GFP YTA

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAAAG

Plasmid

#170096

Purpose

deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAATG

Plasmid

#170097

Purpose

deGFP reporter plasmid with CAATG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTAAT

Plasmid

#170098

Purpose

deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP-HDEL

Plasmid

#136097

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-ST*-GFP

Plasmid

#163669

Purpose

Expresses FKBP tagged partial length ST in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)

Use

Tags

FK506 binding protein (FKBP) and GFP

Expression

Mammalian

Mutation

This chimera contains the first 113 amino acids o…

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4b-EGFP

Plasmid

#176011

Purpose

Mammalian expression of zebrafish Cavin4b-EGFP. Parton lab clone HXM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (cavin4b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-CT-Tagging

Plasmid

#165871

Purpose

Template for amplification of EGFP C-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - CT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.EFS.eGFP.mArid3a-3'UTR-WT

Plasmid

#169299

Purpose

Constitutive overexpression of the 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3'UTR-WT (Arid3a Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP

Plasmid

#175235

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

Plasmid

#169316

Purpose

Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.GFP.miR-ctrl

Plasmid

#169305

Purpose

Constitutive overexpression of miR-ctrl with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-NT-Tagging

Plasmid

#165868

Purpose

Template for amplification of EGFP N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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