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We narrowed to 29,304 results for: uros

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AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171595

Purpose

Weak human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Histone H2B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45216

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

Histone H2B-targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

Plasmid

#138143

Purpose

Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PguCas13b

Use

Lentiviral

Tags

NLS

Expression

Mutation

Promoter

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Use

Tags

3xGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

Plasmid

#184952

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Lacra Bintu, Michael Bassik, Ami Bhatt, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

Plasmid

#171965

Purpose

Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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1072 pBabe puroL myr Akt K179M T308A S473A

Plasmid

#9015

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

K179M T308A S473A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216251

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgSEC61B.N1 (SEC61B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA

Plasmid

#191685

Purpose

For knocking p2a-tdTomato into CLSTN2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

CLSTN2 homology arms and p2a-tdTomato

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

Plasmid

#122202

Purpose

Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

Depositor

Raphael Gaudin

Article

Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

Inserts

HBc from HBV AYW
mNeonGreen

Use

Tags

GGSGGSGGS linker

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

Plasmid

#138144

Purpose

Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PguCas13b

Use

Lentiviral

Tags

NES

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

Plasmid

#47458

Purpose

LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro

Plasmid

#25955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25957

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

Plasmid

#132401

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

Plasmid

#132409

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRanCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H147A; H1044A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

Plasmid

#132408

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLbaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R600A; H605A; R1243A; H1248A

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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