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Plasmid#154012PurposeExpresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutation381-518 fragmentPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 K46I
Plasmid#27627DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseRetroviralTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 UQCC2(5’UTR)-FF
Plasmid#85487PurposeFirefly luciferase under the control of UQCC2 5'UTRDepositorInsert5'UTR of UQCC2 (C6orf125 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-Y619E-FRAG
Plasmid#68871PurposeMammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutationDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAGExpressionMammalianMutation331-672aa, Y619EPromoterCMVAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
fox1 (a2bp1l)_R (OZ520)
Plasmid#27195DepositorInsertZinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
fox1 (a2bp1l)_L (OZ519)
Plasmid#27194DepositorInsertZinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag-SKP1-T131A
Plasmid#213734PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-113-117-5A
Plasmid#213643PurposeExpresses human SKP1-113-117-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-113-117-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-138-142-5A
Plasmid#213645PurposeExpresses human SKP1-138-142-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-138-142-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-120-124-5A
Plasmid#213644PurposeExpresses human SKP1-120-124-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-120-124-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-RR (K142R/K163R)
Plasmid#213425PurposeExpresses human SKP1 K142R/K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R/K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-F139A/I141A
Plasmid#213687PurposeExpresses human SKP1-F139A/I141A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-F139A/I141A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGBacmam-NGS Wnt-Fc
Plasmid#159629PurposeExpress Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid researchDepositorInsertFzd7/8 subtype NGS Wnt Fc
UseTagsFc tag and His tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorUseTagsExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7 (R310H)
Plasmid#171849PurposepcDNA3-FLAG_FBXL7 (R310H)DepositorInsertF-box and leucine rich repeat protein 7 (FBXL7 Human)
UseTagsFlagExpressionMammalianMutationchanged Arginine 310 to HistidinePromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(TISU)-FF
Plasmid#85489PurposeFirefly luciferase under the control of ATP5O TISUDepositorInsertFull TISU element of ATP5O (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutation2nd and 3rd codon of luciferase were modified fro…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(5’UTR)-SL-FF
Plasmid#85491PurposeFirefly luciferase under the control of ATP5O 5'UTR followed by a stem-loopDepositorInsert5'UTR of ATP5O followed by stem loop (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]
Plasmid#197599PurposePan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactiveDepositorUseTagsExpressionWormMutationD387A, E490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only