Addgene

We narrowed to 3,692 results for: c1s

Showing: 3401 - 3420 of 3692 results

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KCC2-pHext

Plasmid

#104076

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

pHluorine tag inserted in the second putative tra…

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-N-terminus(1-111)

Plasmid

#64671

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only N-terminus amino acids 1 to 111

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-cdc42palm

Plasmid

#20141

Depositor

Alaa El-Husseini

Article

Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Insert

cell division cycle 42 isoform 2 (CDC42 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pTE4560

Plasmid

#107526

Purpose

Expresses As crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH-ITSN1

Plasmid

#176113

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pmKikGR Actin

Plasmid

#105315

Purpose

Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

actin beta (ACTB Human)

Use

Tags

KikGR

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-cdc42prenyl

Plasmid

#20142

Depositor

Alaa El-Husseini

Article

Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Insert

cell division cycle 42 isoform 1 (CDC42 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCMV AURKA-mTurq2

Plasmid

#157767

Purpose

Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al Elife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mRuby2-Actin-C-18

Plasmid

#55889

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Actin (ACTB Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
5HT6-YFP-ThymosinBeta4(KK18,19EE)

Plasmid

#96807

Purpose

non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

KK18,19EE prevents G-actin binding

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-C-terminus

Plasmid

#64673

Purpose

expresses only C terminus of RNF8, amino acids 402-485

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only C-terminus amino acids 402 to 485

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
mEmerald-MyosinIIC-C-18

Plasmid

#54194

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Mouse GFP-PRMT7 Wild Type

Plasmid

#65056

Purpose

Expresses wild type mouse GFP-PRMT7

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
mIFP12-ZO1-C-14

Plasmid

#56265

Purpose

Localization: Tight Junctions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

ZO1 (TJP1 Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits