Addgene

We narrowed to 10,631 results for: GAN

Showing: 3401 - 3420 of 10631 results

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_R (OZ520)

Plasmid

#27195

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_L (OZ519)

Plasmid

#27194

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pBabe-Flag-SKP1-T131A

Plasmid

#213734

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-113-117-5A

Plasmid

#213643

Purpose

Expresses human SKP1-113-117-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-113-117-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-138-142-5A

Plasmid

#213645

Purpose

Expresses human SKP1-138-142-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-138-142-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-120-124-5A

Plasmid

#213644

Purpose

Expresses human SKP1-120-124-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-120-124-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-RR (K142R/K163R)

Plasmid

#213425

Purpose

Expresses human SKP1 K142R/K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R/K163R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-F139A/I141A

Plasmid

#213687

Purpose

Expresses human SKP1-F139A/I141A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-F139A/I141A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Use

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Use

Tags

Fc tag and His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet_huCAD_ICADL (TevS)

Plasmid

#100098

Purpose

dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

DNA fragmentation factor subunit alpha (CAD, DFFA Human)

Use

Tags

Expression

Bacterial

Mutation

two caspase cleavage sites (DETD-117 and DAVD-224…

Promoter

T7

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Article

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(TISU)-FF

Plasmid

#85489

Purpose

Firefly luciferase under the control of ATP5O TISU

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

Full TISU element of ATP5O (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

2nd and 3rd codon of luciferase were modified fro…

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 ATP5O(5’UTR)-SL-FF

Plasmid

#85491

Purpose

Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of ATP5O followed by stem loop (ATP5PO Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

Plasmid

#197599

Purpose

Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)

Use

Tags

Expression

Worm

Mutation

D387A, E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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