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pSLIK 3xFLAG-PFN2_variant2 zeo

Plasmid

#192325

Purpose

Lentiviral expression vector for an inducible 3xFLAG-PFN2_variant2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

PFN2_variant2 (PFN2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MTUS1

Plasmid

#192298

Purpose

Gateway entry vector for an inducible 3xFLAG-MTUS1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

MTUS1 (MTUS1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-NUP37

Plasmid

#192299

Purpose

Gateway entry vector for an inducible 3xFLAG-NUP37

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NUP37 (NUP37 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-PFN2_variant2

Plasmid

#192300

Purpose

Gateway entry vector for an inducible 3xFLAG-PFN2_variant2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

PFN2_variant2 (PFN2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-TTI1

Plasmid

#192303

Purpose

Gateway entry vector for an inducible 3xFLAG-TTI1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

TTI1 (TTI1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Use

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-N-DiB2

Plasmid

#168473

Purpose

N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2 + Leucine Zipper

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab38a

Plasmid

#140887

Purpose

Expression of zebrafish GFP-tagged Rab38a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab38a (rab38a Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p8195 MSCV-P C-FlagHA 6E7 R77S

Plasmid

#163309

Purpose

Expresses HPV6 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV6 E7 R77S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p8194 MSCV-P C-FlagHA 31E7 R77S

Plasmid

#163308

Purpose

Expresses HPV31 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV31 E7 R77S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG-altTAG

Plasmid

#158146

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG

Plasmid

#158145

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BYG

Plasmid

#158144

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (wild-type)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p-FAPB2.3.6

Plasmid

#158142

Purpose

Yeast display reporter for non-display yeast strains (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

FAPB2.3.6

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158143

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

FAPB2.3.6L1TAG

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p8116 LentiCRISPR v2 sgPTPN14-4

Plasmid

#163316

Purpose

Expresses Cas9 and a human PTPN14-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgPTPN14-4

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p8220 MSCV-Neo C-HA 18E7 R84S

Plasmid

#163312

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

HA

Expression

Mutation

R84S

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p8193 MSCV-P C-FlagHA 18E7 R84S

Plasmid

#163307

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

FlagHA

Expression

Mutation

R84S

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Use

Tags

Avi and Flag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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