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Plasmid#13321DepositorInsertPINK1 kinase domain (PINK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 112-496PromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
NAE1 G8.3 gRNA
Plasmid#90777Purpose3rd generation lentiviral gRNA plasmid targeting human NAE1DepositorInsertNAE1 (Guide Designation G8.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
NAE1 G7.3 gRNA
Plasmid#90776Purpose3rd generation lentiviral gRNA plasmid targeting human NAE1DepositorInsertNAE1 (Guide Designation G7.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Puro-iNotch1IC
Plasmid#75338Purposedoxycycline inducible Notch1 intracellular domain gene over-expressionDepositorInsertNotch1 Intracellular domain (Notch1 Mouse)
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP
Plasmid#199358PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Golgi-ABKAR
Plasmid#61507PurposeExpression of AMPK biosensor targeted to the GolgiDepositorInsertAMPK Biosensor
UseTagsGolgi-targeting signal (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-ABKAR
Plasmid#65068PurposeExpression of ABKAR biosensor targeted to the lysozomeDepositorInsertABKAR biosensor
UseTagsLamp1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3C
Plasmid#105047PurposeOverexpression of APOBEC3C in mammalian cell linesDepositorInsertAPOBEC3C (A3C) (APOBEC3C Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-GATA6
Plasmid#51929Purposemammalian expression of mouse GATA6DepositorInsertGATA6 (Gata6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pV92.2
Plasmid#78409PurposepKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron constructDepositorInsertsRT-Cas1
Cas2
UseTagsExpressionMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CHEK2 C3.2 gRNA
Plasmid#90628Purpose3rd generation lentiviral gRNA plasmid targeting human CHEK2DepositorInsertCHEK2 (Guide Designation C3.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHEK1 C1.2 gRNA
Plasmid#90626Purpose3rd generation lentiviral gRNA plasmid targeting human CHEK1DepositorInsertCHEK1 (Guide Designation C1.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS
Plasmid#199347PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKSJ376
Plasmid#103065PurposeExpresses colicin E1 with R domain deletion and ImmE1DepositorUseTagsExpressionBacterialMutationR (receptor-binding domain) deletion in colicin E…PromoterT7 lac (from pET) and T7 lac (from pET52-b)Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP
Plasmid#199363PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only