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Plasmid#27366DepositorInsertPmyc1tetO
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNR-DEST
Plasmid#90101PurposeGateway destination vector featuring nitrate reductase (NR) promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.DepositorTypeEmpty backboneUseGateway destination vector for protein expression…TagsExpressionMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH4-DEST
Plasmid#90099PurposeGateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.DepositorTypeEmpty backboneUseGateway destination vector for protein expression…TagsExpressionMutationPromoterH4Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
371 pTol2-fli1ep:MTDest
Plasmid#73490PurposeTol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signalDepositorInsertfli1ep:6xMyc-attR1-attR2
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC+ATG
Plasmid#49323PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
373 pTol2-fli1ep:CherryDest
Plasmid#73493PurposeTol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signalDepositorInsertfli1ep:mCherry-attR1-attR2
UseTagsExpressionMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONRCBX
Plasmid#29634DepositorTypeEmpty backboneUseGateway donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-DEST-His
Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStart-K
Plasmid#20346DepositorTypeEmpty backboneUseMouse Targeting; Gateway cloningTagsExpressionMutationPromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEG545
Plasmid#40116DepositorTypeEmpty backboneUseGateway donor vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionMutationPromoterAvailable sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-Myc
Plasmid#119752PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
372 pTol2-fli1ep:EGFPDest
Plasmid#73491PurposeTol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signalDepositorInsertfli1ep:EGFP-attR1-attR2
UseTagsExpressionMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN41A-T10M
Plasmid#27365DepositorInserttetR(sB)
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceJune 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGGW_SM_BamHI
Plasmid#49315PurposeSubcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insertDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONRHYG
Plasmid#29636DepositorTypeEmpty backboneUseGateway donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pBSWt1-Dest
Plasmid#59764PurposePCR template for amplifying the attL1-em7-kanr-attL2-pA cassetteDepositorInsertattL1-em7-kanR-attL2-pA
UseTagsExpressionMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -