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Plasmid#197596PurposePan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH
Plasmid#131684PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHExpressionMutationPromoterEF1a and mU6-LSL (Cre dependent)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K142R
Plasmid#213423PurposeExpresses human SKP1 K142R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131A
Plasmid#213482PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-97-101-5A
Plasmid#213642PurposeExpresses human SKP1-97-101-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1--97-101-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-K163R
Plasmid#213424PurposeExpresses human SKP1 K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-T131E
Plasmid#213601PurposeExpresses human SKP1 T131E mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131E (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV06 [punc-17::SNG-1::CRY2(535)]
Plasmid#197597PurposeExpression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)
Plasmid#155010PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag(A227Y)
Plasmid#155013PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA227YPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-CHD5-L518A/Y619E-FRAG
Plasmid#68874PurposeIPTG inducible bacterial expression of CHD5 fragmentDepositorInsertCHD5 (CHD5 Human)
UseTagsGSTExpressionBacterialMutation331-672aa, L518A/Y619EPromotertacAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7
Plasmid#171848PurposepcDNA3-FLAG_FBXL7DepositorInsertF-box and leucine rich repeat protein 7 (FBXL7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorInsertJag1 (Jag1 Rat)
UseTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(5’UTR)-FF
Plasmid#85486PurposeFirefly luciferase under the control of ATP5O 5'UTRDepositorInsert5'UTR of ATP5O (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
Plasmid#128833PurposeKnock-in of nuclear EYFP into mouse Elf5 locusDepositorInsertNuclear EYFP into Elf5 locus (Elf5 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 4SA
Plasmid#27628DepositorInsertmouse myc ULK1 4SA (Myc Mouse)
UseRetroviralTagsmycExpressionMutationS467A, S555A, T574A, S637A. Initial M from ULK1 r…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only