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Plasmid#193575PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ShadowY
Plasmid#193555PurposeExpresses mEGFP-ShadowY in mammalian cells.DepositorInsertShadowY
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CLCdeltaN
Plasmid#193562PurposeExpresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 1-89PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO
Plasmid#195495PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17DepositorInsertsgRNA (SOX17 Human)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterU6Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_dGFPmut3-LVA
Plasmid#187378PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a LVA C-terminal tail)DepositorInsertdGFPmut3_LVA
UseTagsLVAExpressionBacterialMutationC-terminal LVA tailPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Letm1
Plasmid#191937PurposeExpression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.DepositorInsertLetm1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP129
Plasmid#189537PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_GFP11-14-SEPT7
Plasmid#180342Purposemammalian expression of human SEPT7 fused to GFP11DepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-14-GFP11
Plasmid#180344Purposemammalian expression of human SEPT9_i1 fused to GFP11DepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3-14-GFP10
Plasmid#180345Purposemammalian expression of human SEPT9_i3 fused to GFP10DepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsGFP10ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3-14-GFP11
Plasmid#180346Purposemammalian expression of human SEPT9_i3 fused to GFP11DepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-RvCAHS12-GS
Plasmid#192462PurposeExpression of RvCAHS12-GS_linker-AcGFP1 in mammalian cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_eGFP
Plasmid#180379Purposelentiviral transduction of eGFP geneDepositorInserteGFP (eGFP )
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSV40_mD6Ertd527e-HA-EGFP
Plasmid#192223PurposeExpresses murine mD6Ertd527e gene, C-terminally tagged with HA and EGFP, in mammalian cellsDepositorInsertmD6Ertd527e (D6Ertd527e Mouse)
UseTagsHA-EGFPExpressionMammalianMutationPromoterSV40Available sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPT-mEGFP
Plasmid#190606PurposeObtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)DepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationa deletion was created in mCherry CDS impairing i…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-TetO-GFP_ColE1
Plasmid#173193PurposeExpresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.DepositorInsertT7-TetO
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-10-QUAS-GFP_ColE1
Plasmid#171657PurposeQUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertT7-10-QUAS
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-15-QUAS-GFP_ColE1
Plasmid#171658PurposeQUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertT7-15-QUAS
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only