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Plasmid#102459PurposeExpresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ1AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-SZ158a-sfGFP
Plasmid#162446PurposeExpression in HEK293T cell and compete ligand singaling against full-length receptorsDepositorInsertC-X-C chemokine receptor type 4 (CXCR4 Human)
UseTagssfGFPExpressionMammalianMutationtruncation from aa52 to aa257PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
PB_EF1a-DjCas13d-msfGFP-Blast
Plasmid#226008PurposeDjCas13d protein for RNA targeting in piggyBac backboneDepositorInsertDjCas13d RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC034 - LwCas13a-msfGFP-2A-Blast
Plasmid#91924PurposeExpresses active LwCas13a for mammalian RNA targeting with Blasticidin selection markerDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNLS and msfGFP-NLS-3xHA, P2A, BlasticidinExpressionMammalianMutationPromoterEFSAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sfGFP-iCre-ERT2
Plasmid#125749PurposesfGFP and iCreERT2 fused by unskippable linkerDepositorInsertsfGFP-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-sfGFP-T2A-iCreERT2
Plasmid#177309PurposeCAG-sfGFP-T2A-iCre-ERT2 piggyBac transposonDepositorInsertsfGFP-T2A-iCreERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0056 - LwCas13a-msfGFP-NES
Plasmid#105815PurposeExpresses active LwaCas13a-NES for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNES and msfGFP-NES-3xHAExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*- MmH-MH4R
Plasmid#196409PurposeExpress sfGFP with TAG and enzymes for biosynthesizing DOPADepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-134/150TAG
Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-TAG35-sfGFP
Plasmid#212120PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-TAG35-sfGFP
M. alvus Pyl-tRNA
UseTagsHis6 and SUMO-TAG35ExpressionBacterialMutationE35TAG (within SUMO encoding sequence)PromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6/sgClta-sfGFP-Clta
Plasmid#200261PurposeU6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.DepositorInsertsfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MCS-P2A-sfGFP
Plasmid#74668PurposePlasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.DepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
UseTagsExpressionBacterialMutationPromoterPRHIIIAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETduet+sfGFP-TetR-p300HAT
Plasmid#157795PurposeExpression of sfGFP-TetR-p300HAT fusion proteinDepositorInsertsfGFP-TetR-p300 (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_YB TATA-sfGFP-CMV_dsRed
Plasmid#117399PurposesfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-sfGFP-TET1CD
Plasmid#184439PurposeTET1 catalytic domain fused with sfGFP and scFv against GCN4DepositorInsertscFv-GCN4-sfGFP-TET1CD
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only