Addgene

We narrowed to 3,627 results for: sam

Showing: 321 - 340 of 3627 results

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAct:MS2-p65-HSF

Plasmid

#78902

Purpose

Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-p65-HSF

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK GSC 3'UTR

Plasmid

#19859

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

GSC 3'UTR (GSC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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SuExp-mPLD3

Plasmid

#201245

Purpose

express full length mouse PLD3 protein

Depositor

David Nemazee

Article

Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

Insert

PLD3 (Pld3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180267

Purpose

Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

EGFR sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SuExp His-Myc-mPLD3

Plasmid

#173853

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD3

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD3 (Pld3 Mouse)

Use

Tags

6xHis, Myc, Factor X cleavable

Expression

Mammalian

Mutation

intracellular and transmembrane domain truncated,…

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180268

Purpose

Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

GARP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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MoFGFR2b-ires neo

Plasmid

#86491

Purpose

mamalian cell expression of FGFR2b

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIb 13A4 (FGFR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2317

Plasmid

#36743

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Right (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2316

Plasmid

#36742

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Left (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only
MAC3-FGFR1

Plasmid

#222611

Purpose

Expression of MAC3-tagged FGFR1

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

FGFR1 (FGFR1 Human)

Use

Tags

MAC3

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TAL2317

Plasmid

#36743

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Right (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2316

Plasmid

#36742

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FGFR1-TALEN_Left (FGFR1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1600

Plasmid

#29274

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple129 (MKI67 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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FGFR1-optoDroplet

Plasmid

#111509

Purpose

For optogenetic clustering of FGFR1

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Tags

Cry2, FUS, Fusion Red, and Myristoylation sequenc…

Expression

Mutation

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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SuExp His-Myc-mPLD4

Plasmid

#201185

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD4

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD4 (Pld4, Pld4, Pld4 Mouse)

Use

Tags

His-Myc, Factor Xa cleavable

Expression

Mammalian

Mutation

cytosolic and transmembrane domain truncated; add…

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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