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Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX551-miniCMV-SaCas9
Plasmid#107032PurposeAAV plasmid expressing SaCas9 under control of miniCMV promoterDepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-CjCas9
Plasmid#107033PurposeAAV plasmid expressing CjCas9 under control of miniCMV promoterDepositorInsertCjCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CBh-Null-WPRE3-enSV40pA
Plasmid#194245Purposeempty backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceJan. 27, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAM/SAR-CAG-WPRE-bGHpA
Plasmid#194243Purposeempty backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceJan. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Top7 K39E K40E V48V K55E
Plasmid#12464PurposePlasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.DepositorInsertTop7 K39E K40E V46A K55E
UseTags6xHisExpressionBacterialMutationK39E K40E V46A K55EPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAVtri-CMV-eGFP
Plasmid#230932PurposeUsed for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
Plasmid#51690PurposeYeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisGDepositorInserthisG-URA3-hisG (URA3 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lourido Toxoplasma CRISPR Library V1
Pooled Library#80636PurposePlasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.DepositorAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
XA106+pRCPam
Bacterial Strain#195858PurposeE. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
XA103+pRCPam
Bacterial Strain#195857PurposeE. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
XA101+pRCPam
Bacterial Strain#195856PurposeE. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
XA92+pRCPam
Bacterial Strain#195855PurposeE. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.DepositorBacterial ResistanceChloramphenicolSpeciesAcropora milleporaAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
Plasmid#175570PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. G to C …PromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBScrispri Sham
Plasmid#223201PurposeGroup B Streptococcus inducible dCas12 CRISPRi with Modular crRNADepositorInsertN/A
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601 miniCMV-SaCas9-SpA-sgRNA scaffold
Plasmid#107055PurposeBackbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only