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Plasmid#68438PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSico-shRheb
Plasmid#81087Purposeconditional expression of an shRNA targeting murine RhebDepositorInsertRheb shRNA (Rheb Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[P4-P6(3xPP7)]
Plasmid#68432PurposeTransient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginC
Plasmid#29505DepositorInsertElongin C (ELOC Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYR
Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-EloC
Plasmid#31982DepositorInsertElongin C (ELOC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-EGFP-Rheb-HVR HRas-IP
Plasmid#13833DepositorInsertRheb-HVR HRas (Rheb Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationN-terminal of Rheb and hyper variable region (HVR…PromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_R4187C
Plasmid#187141PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationR4187CPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_F3194S
Plasmid#187139PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationF3194SPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_H669Q
Plasmid#187137PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationH669QPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_H3962D
Plasmid#187130PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationH3962DPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NEB-1/1
Plasmid#91396PurposeProtein expression and purification of human SH3 domain construct NEB-1/1DepositorInsertNEB-1/1 (NEB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_FLAG-HUWE1
Plasmid#187121Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_C4341S
Plasmid#187156Purposemammalian cell expression FLAG-HUWE C4341SDepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationC4341SPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Dnase1L3 pIRES-DsRed
Plasmid#98845PurposeExpresses Human Dnase1L3 in mammalian cells along with DsRedDepositorInsertDnase1L3 (DNASE1L3 Human)
UseTagsIRES DsRedExpressionMammalianMutationlong isoform, wild typePromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only