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Showing: 321 - 340 of 10835 results

  1. SOAP-COMP-blac-flag-his

    Plasmid
    #110997
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110997-plasmid-map-sequence-id-224488

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  2. CTRP-COMP-blac-flag-his

    Plasmid
    #110994
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    circumsporozoite- and TRAP-related protein (CTRP) (CTRP Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110994-plasmid-map-sequence-id-224485

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  3. P25-COMP-blac-flag-his

    Plasmid
    #110991
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    ookinete surface protein P25 (PF10_0303 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110991-plasmid-map-sequence-id-224481

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  4. WARP-COMP-blac-flag-his

    Plasmid
    #110989
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    von Willebrand factor A-domain related protein (WARP) (PF08_0136b Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110989-plasmid-map-sequence-id-224479

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  6. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  7. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  8. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid
    #110376
    Purpose
    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110376-plasmid-map-sequence-id-213402

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  10. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

    Plasmid
    #110830
    Purpose
    Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    AZGP1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    T2A and dTomato
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110830-plasmid-map-sequence-id-214108

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  13. pBABE.zeo/TR

    Plasmid
    #110358
    Purpose
    Mammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systems
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Tetracycline repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    gag truncated
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110358-plasmid-map-sequence-id-213700

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  17. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110426-plasmid-map-sequence-id-213399

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  18. mCherry-PLEKHS1

    Plasmid
    #110510
    Purpose
    Fluorescent PLEKHS1
    Depositor
    Bernardo Mainou
    Insert
    PLEKHS1 (C10orf81 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110510-plasmid-map-sequence-id-210717

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Showing: 321 - 340 of 10835 results